Re: alcohol-based fixation for PCNA detection

From:Albert Grobe

We have used Histochoice fixative with good success in paraffin-embedded sheep tissues when staining for PCNA.

>>> Kristel Wautier  09/18/02 10:43AM >>>
Hello everybody,

I have recently read that PCNA is found in two fractions in cells: a fraction associated with the chromosomes and a fraction free in the nucleoplasm. The latter can be "eliminated" by using an alcohol-based fixative, prior to immunohistochemical detection of PCNA.

Does anybody have any suggestions on which fixative would be best. I have been thinking of methacarn. Any suggestions or comments on the quality of morphology when using alcohol-based fixatives?


Kristel Wautier
Ghent University
Department of Biology
K.-L. Ledeganckstraat 35
9000 Ghent

Tel.: +32 9 264 52 31
Fax: +32 9 264 53 44 

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