I just read the response about the person studying with the Frieda Carlson
book. I'm just starting to study and I have that plus the old Dezna Sheehan
book. What is the best study guide to have? Is it the one from the Michigan
State Society or is it NSH or Sheehan? I don't want to study in vain.
Please give me some suggestions and if anyone has info on the Michigan
study guide can you post it. Thanks. A newbie.

--- Felisha Sandifer
--- leish27@earthlink.net
--- EarthLink: The #1 provider of the Real Internet.


> [Original Message]
> From: HistoNet Server 
> To: HistoNet Server 
> Date: 9/28/2002 1:00:05 AM
> Subject: Daily Digest
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 00:16:14 -0500
> From: "Brothers, Julie {CNS~Palo Alto}" 
> Subject: Out of Office AutoReply: Daily Digest
>
> Hello -  I am currently out of the office and will be back on Monday,
> September  30th.   I will not be checking my email. 
>   Please see Renee Martin for any urgent matters.
> Thanks, Julie
>  
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 00:30:43 -0500
> From: Jen Wyffels & Greg Shafer 
> Subject: Repair MT2 Microtome
>
>
> Is there anyone available to service the Sorvall MT2 microtome?  I have
> contacted many companies including Ventana, Leica, Boeckeler, ect...
without
> any luck.  Thanks  Jennifer
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
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> 
> 
> 
> 
> 
> 
> 
> 
Is there anyone available to service the
Sorvall 
> MT2 microtome?  I have contacted many companies including Ventana,
Leica,
>
> Boeckeler, ect... without any luck.  
> Thanks  Jennifer
>
> - --Boundary_(ID_zBmE5IFhlfrDud5nBu2QKg)--
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 01:00:44 -0500
> From: HCS 
> Subject: looking for part
>
>
> I have a Microm HM330 and I am looking for parts for the disposable knife
> holder,  the part that holds the blade tight.  Does anyone have one of
these
> parts or know who to contact.   Thanks in advance.
>
> LeRoy Brown HT(ASCP) HTL
> Histology Consultation Services
> www.Histocs.com
> Everson, WA 98247
> 1-800-732-8158
> rbrown@alphahunt.com
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This is a multi-part message in MIME format.
>
> - --Boundary_(ID_CDxxoCgIC4uLzjb35+6eqg)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_CDxxoCgIC4uLzjb35+6eqg)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> 
> 
> 
> 
> 
> 
> 
> 
I have a Microm HM330 and I am looking for
parts 
> for the disposable knife holder,  the part that holds the blade 
> tight.  Does anyone have one of these parts or know who to 
> contact.   Thanks in advance.
> 
 
> 
LeRoy Brown HT(ASCP) HTL
> 
Histology Consultation Services
> 
 href="http://www.Histocs.com">www.Histocs.com
> 
Everson, WA 98247
> 
1-800-732-8158
> 

href="mailto:rbrown@alphahunt.com">rbrown@alphahunt.com
>
> - --Boundary_(ID_CDxxoCgIC4uLzjb35+6eqg)--
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 01:16:02 -0500
> From: Andi Kappeler 
> Subject: Re: protein gene product 9.5
>
> Hi Annette
>
> We use mo-a-PGP 9.5, clone 13C4, Biogenesis 7863-2004 or Biomeda U3231
(dil.
> 1:500 and 1:100, respectively). HIER required (citrate buffer pH 6.0,
> microwave) for FFPE tissue (3-5 um sections), used on various tissue
types;
> regular 3-step IHC (ABC, LSAB) on slides, no floating sections. Polyclonal
> (rb) PGP 9.5 Abs available e.g. from Dako (no personal experience with
this
> one). Good luck!
>
> Andi Kappeler
> Institute of Pathology, University of Bern, Switzerland
>
> - ----- Original Message -----
> From: "Featherstone, Annette" 
> To: 
> Sent: Thursday, September 26, 2002 7:32 PM
> Subject: protein gene product 9.5
>
>
> > Is anyone working with Protein Gene Product 9.5?  What type of tissue
are
> > you using? Is it a 50u section? Do you float it in antibody?  Does
anyone
> > have a protocol?
> >
> > Thanks
> > Annette MLT/HT
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 01:30:59 -0500
> From: "R.Z. Meitus" 
> Subject: Re: protein gene product 9.5
>
> Annette,
> I have used this antibody for skin sections to
> demonstrate peripheral nerves.  I did the sectioning
> on a sliding microtome @ 50um for a 3mm skin punch bx.
> I used 96 well tissue culture plates (on a rocker) to
> float the sections in the reagents.  I do have a
> protocol that I will hapilly share BUT I will be at
> the National meeting in Long Beach and probably won't
> be able to fax it until next Friday.
>
>
>
> =====
> Rebecca Z. Meitus, HTL
> Vanderbilt University Medical Center
> Department of Pathology
> Division of Neuropathology
>
> __________________________________________________
> Do you Yahoo!?
> New DSL Internet Access from SBC & Yahoo!
> http://sbc.yahoo.com
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 06:30:09 -0500
> From: "Molinari, Betsy" 
> Subject: RE: written portion of HT
>
> The Michigan Society of Histology publishes this fantastic workbook that I
> would HIGHLY recommend. I do not have the ordering information available
but
> I am sure someone will post it for you.  I don't think I would have done
so
> well if I had not used it. Also, use more than one text and the question
and
> answer books that the NSH offers are very good too. 
> Betsy Molinari HT(ASCP)
> Texas Heart Institute
> Houston, TX 77030
> 832-355-6524
> 832-355-6812 (fax)
>
> - -----Original Message-----
> From: Travelinjill@aol.com [mailto:Travelinjill@aol.com] 
> Sent: Thursday, September 26, 2002 9:41 PM
> To: histonet@pathology.swmed.edu
> Subject: written portion of HT
>
> I just took the written portion of the HT exam and did not recognize
hardly
> any questions that I have studied. I used the Histotechnology a self inst.
> text by Freida Carson along with the work book. Is there a better
reference
> out there I can get just in case I have to take it again? Please help,
> thanks
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 06:30:23 -0500
> From: marjorie lehman 
> Subject: CD71
>
> Does anyone have a protocol for CD71 on frozen or FFPE skin? I have tried
one
> prediluted Ab ("suitable for both frozen & FFPE") on both, have done HIER,
> trypsin digestion, 1 hour,  2 hour and overnight incubation and haven't
gotten
> anywhere. I really don't want to start buying Abs that don't work for me,
so
> any help would really be appreciated.
> Marge Lehman
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 07:01:03 -0500
> From: Jackie.O'Connor@abbott.com
> Subject: Re: Repair MT2 Microtome
>
>
> Try Ken Hart at Dorn and Hart Microedge in Villa Park, IL. PH:
630-832-3843.
> I've shipped machines to him in the past for repair - he does excellent
work.
>
>
>
>                                                                          

>                                 
>                     Jen Wyffels &                                        

>                                 
>                     Greg Shafer          To:    
histonet@pathology.swmed.edu 
>                                 
>                                                      
>                     bb.ne.jp>            Subject:     Repair MT2
Microtome    
>                                 
>                                                                          

>                                 
>                     09/27/2002                                           

>                                 
>                     12:17 AM                                             

>                                 
>                                                                          

>                                 
>                                                                          

>                                 
>
>
>
>
> Is there anyone available to service the Sorvall MT2 microtome?  I have
> contacted many companies including Ventana, Leica, Boeckeler, ect...
without
> any luck.  Thanks  Jennifer
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 07:15:44 -0500
> From: Kristin Stuke 
> Subject: feline monocyte-derived macrophages
>
> Does anybody know a method on how to acquire monocyte-derived 
> macrophages from cat PBMC?
>
> Thanks,
>
> Kristin Stuke
> School of Vet. Med. 
> Hannover, Germany
> kristin.stuke@tiho-hannover.de
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 07:16:04 -0500
> From: Robert Schoonhoven 
> Subject: Re: looking for part
>
>
> Richard Allan Scientific 800-522-7270 now owns/distributes Microm
>
> HCS wrote:
>
> > I have a Microm HM330 and I am looking for parts for the disposable
> > knife holder,  the part that holds the blade tight.  Does anyone have
> > one of these parts or know who to contact.   Thanks in advance. LeRoy
> > Brown HT(ASCP) HTLHistology Consultation
> > Serviceswww.Histocs.comEverson, WA
> > 982471-800-732-8158rbrown@alphahunt.com
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This is a multi-part message in MIME format.
>
> - --Boundary_(ID_xOQ9I2iBPD6z0GI7DEN0Tw)
> Content-type: multipart/alternative;
>  boundary="Boundary_(ID_MS865Llontd72BBUoi6FeQ)"
>
>
> - --Boundary_(ID_MS865Llontd72BBUoi6FeQ)
> Content-type: text/plain; charset=us-ascii
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_MS865Llontd72BBUoi6FeQ)
> Content-type: text/html; charset=us-ascii
> Content-transfer-encoding: 7BIT
>
> 
> 
> 
> Richard Allan Scientific 800-522-7270 now owns/distributes Microm
> 
HCS wrote:
> 

> I
> have a Microm HM330 and I am looking for parts for the disposable knife
> holder,  the part that holds the blade tight.  Does anyone have
> one of these parts or know who to contact.   Thanks in
> advance. LeRoy
> Brown HT(ASCP) HTLHistology
> Consultation Services href="http://www.Histocs.com">www.Histocs.com face="Arial">Everson,
> WA 98247 size=-1>1-800-732-8158
href="mailto:rbrown@alphahunt.com">rbrown@alphahunt.com
>
> 
> 
>
> - --Boundary_(ID_MS865Llontd72BBUoi6FeQ)--
>
> - --Boundary_(ID_xOQ9I2iBPD6z0GI7DEN0Tw)
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> Content-description: Card for Robert Schoonhoven
>
> begin:vcard 
> n:Schoonhoven;Robert
> tel;fax:919-966-6123
> tel;work:919-966-6343
> x-mozilla-html:FALSE
> org:University of North Carolina;Environmental Sciences & Engineering
> adr:;;CB#7431;Chapel Hill;NC;27599-7431;United States
> version:2.1
> email;internet:robert_schoonhoven2unc.edu
> fn:Robert Schoonhoven
> end:vcard
>
> - --Boundary_(ID_xOQ9I2iBPD6z0GI7DEN0Tw)--
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 09:00:38 -0500
> From: "Charles W. Scouten, Ph.D." 
> Subject: RE: Repair MT2 Microtome
>
> The Vibratome(tm) company will now tackle repairs on any microtome
(rotary,
> sliding or vibratory), cryostat, microforge, tissue embedder, tissue
stainer,
> or other histology equipment item.  See the following link.
>  
>
http://www.myneurolab.com/mnl/mnlsite/ViewProduct.asp?idproduct=610000&catde
sc=Histology+Equipment&subcatdesc=Service&idsubcategory=196
>  
>  
> Cordially,
>  
> Charles W.  Scouten, Ph.D.
> myNeuroLab.com
> 5918 Evergreen Blvd.
> St. Louis, MO 63134
> Ph: 314 522 0300  
> FAX  314 522 0277
> cwscouten@myneurolab.com
> www.myneurolab.com
>  
> - -----Original Message-----
> From: Jen Wyffels & Greg Shafer [mailto:g_shaferjp@ybb.ne.jp] 
> Sent: Friday, September 27, 2002 12:18 AM
> To: histonet@pathology.swmed.edu
> Subject: Repair MT2 Microtome
>  
> Is there anyone available to service the Sorvall MT2 microtome?  I have
> contacted many companies including Ventana, Leica, Boeckeler, ect...
without
> any luck.  Thanks  Jennifer
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 09:30:37 -0500
> From: Travelinjill@aol.com
> Subject: HT Thanks
>
> Just wanted to thank all of you for your response. I will also keep my
fingers
> crossed. You were right, I also couldnt recognize the pictures!! Thanks
again,
> Jill
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 10:30:53 -0500
> From: Martha Ward 
> Subject: C4d staining for renal biopsies
>
> Our lab has had inquiries about adding this antibody to our protocol for
> transplant renal biopsies and I wanted to hear from labs that are
currently
> performing it.  All the protocols I have seen reference frozen tissue but
we
> were wondering if there is a way to do it on ffpe tissues.  Any and all
help
> will be greatly appreciated!
>  
> Martha Ward, MT (ASCP) QIHC
> Wake Forest University Baptist Medical Center
> Winston-Salem, NC
> 1-336-716-2756
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Sep 2002 12:16:01 -0500
> From: "Sebree Linda A." 
> Subject: RE: C4d staining for renal biopsies
>
> Martha,
>
> We only do C4d on frozen renal biopsies  mainly because the clinicians
want
> their answer sooner rather than later.
>
> Linda A. Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-2472
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
>
>
> - -----Original Message-----
> From: Martha Ward [mailto:mward@wfubmc.edu]
> Sent: Friday, September 27, 2002 10:22 AM
> To: HISTONET@pathology.swmed.edu
> Subject: C4d staining for renal biopsies
>
>
> Our lab has had inquiries about adding this antibody to our protocol for
> transplant renal biopsies and I wanted to hear from labs that are
currently
> performing it.  All the protocols I have seen reference frozen tissue but
we
> were wondering if there is a way to do it on ffpe tissues.  Any and all
help
> will be greatly appreciated!
>  
> Martha Ward, MT (ASCP) QIHC
> Wake Forest University Baptist Medical Center
> Winston-Salem, NC
> 1-336-716-2756
>
>
>
> Here are the messages received yesterday!