RE: Cyclin D1
What pH is your retrieval solution? I use a pH 8.0 citrate on my cyclin D1
and it's working pretty well.
From: JPaulB42@aol.com [mailto:JPaulB42@aol.com]
Sent: Wednesday, October 02, 2002 1:40 PM
Subject: Cyclin D1
We are having problems getting a consistent stain with Cyclin D1.
Does anyone have any ideas?
Steam retrieval in EDTA for 40 minutes
Cool for 30 minutes
Primary for 45 minutes
Link for 20 minutes
Strep for 20 minutes
DAB for 5 minutes
Hema for 5 seconds
This procedure is ran on the DAKO Autostainer. Any suggestions would be
appreciated. Thank you.
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