RE: Cyclin D1

From:Bruce Gapinski

Who's the vendor for the primary antibody? I have great results w/ Vector PA
and BioGenex universal kit.
    Bruce (old school, I am not a rep) Gapinski HT(ASCP)

-----Original Message-----
From: []
Sent: Wednesday, October 02, 2002 11:40 AM
Subject: Cyclin D1

Hello again,

       We are having problems getting a consistent stain with Cyclin D1.
Does anyone have any ideas?


Steam retrieval in EDTA for 40 minutes
Cool for 30 minutes
Primary for 45 minutes
Link for 20 minutes
Strep for 20 minutes
DAB for 5 minutes
Hema for 5 seconds

This procedure is ran on the DAKO Autostainer.  Any suggestions would be
appreciated.  Thank you.

Jim Bradford
Orlando, FL 

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