sectioning mouse eyes - help
I have been struggling for a while so I thought I would seek advice/help
I have formalin fixed paraffin embeded mouse eyes I am trying to section on
a Leica microtome at 4-5 microns. I seem to almost invariably get a "hole"
where the lens should be as I get further down the eye, it just seems to
burst open (I know the lens hardens the older the mouse, but there have to
be ways of doing this).
Does anyone have any tricks, tips, advice, anything? I would like to
eventually perform immunohistochemistry on some of the sections and expect
to detect mostly in the lens, hence my dispair.
Columbus Children's Hospital
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