I stain vibratome sections as free-floating IHC. All steps are preformed
with the section floating in solution. Upon completion the sections are
mounted on slides.

Brett

Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Neuroscience
WP26A-3000
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075
e-mail. brett_connolly@merck.com


-----Original Message-----
From: Matt McElwee [mailto:MCELWEE@surgery.wisc.edu]
Sent: Friday, September 13, 2002 5:46 PM
To: histonet@pathology.swmed.edu
Subject: Mounting vibratome sections


Hello Histonetters.  I have a question about doing
immunohistochemistry(IHC) on vibratome sections.  We're using a 16%
gelatin, 30% sucrose embedding medium.  We'd like to be able to do IHC
on these sections, but, of course, they won't stay on the slide when you
put them in solution.  Does anyone know of an embedding medium you can
use that will stick to a slide, or failing that, an easy way to do IHC
on vibratome sections?  Thanks.
Matt 
 
Matt McElwee
Research Specialist
Department of Surgery
K4/617 Clinical Science Center
600 Highland Avenue
Madison, WI 53792
(608) 263-7648
mcelwee@surgery.wisc.edu


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