Re: =?UNKNOWN?Q?May-Gr=FCnwald-Giemsa?=

Hi Agustin,
Giemsa should be easy to destain. Try in  0.05% acetic acid in water.   80%
alcohol may even work.  You will have to dehydrate them afterward.  Make
sure you use methanol before staining.

Rande Kline
EM Science

=?UNKNOWN?Q?Agust=EDn?= Venzano  on 09/06/2002
11:17:24 AM

To:    Histonet Server 
Subject:    =?UNKNOWN?Q?May-Gr=FCnwald-Giemsa?=

Histonetters: I've lately been having some problems with blood films
staining. Despite previously filtering all reagents and strictly following
the required steps the results were frustrating.

Leukocytes' nuclei often appeared faintly stained, and their cytoplasms'
aspect was variable. Sometimes they looked well colored, but often
cytoplasmic coloration resulted dull. Moreover, eosinophils didn't show
there red cytoplasmic granules, instead their cytoplasm appeared rather

Of course I decided to immediately dispose of MG and G solutions in use,
now I need a wise advise of you veteran netters: Many precious foul-stained
slides should be re-stained rather than disposed of. What should I do in
order to:

1. Decolor these slides.
2. Restain them with my new MG and G reagents?

Thank you

Agustin Jose Venzano Halliburton
DVM, INTA, Argentina

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