Giemsa should be easy to destain. Try in 0.05% acetic acid in water. 80%
alcohol may even work. You will have to dehydrate them afterward. Make
sure you use methanol before staining.
=?UNKNOWN?Q?Agust=EDn?= Venzano on 09/06/2002
To: Histonet Server
Histonetters: I've lately been having some problems with blood films
staining. Despite previously filtering all reagents and strictly following
the required steps the results were frustrating.
Leukocytes' nuclei often appeared faintly stained, and their cytoplasms'
aspect was variable. Sometimes they looked well colored, but often
cytoplasmic coloration resulted dull. Moreover, eosinophils didn't show
there red cytoplasmic granules, instead their cytoplasm appeared rather
Of course I decided to immediately dispose of MG and G solutions in use,
now I need a wise advise of you veteran netters: Many precious foul-stained
slides should be re-stained rather than disposed of. What should I do in
1. Decolor these slides.
2. Restain them with my new MG and G reagents?
Agustin Jose Venzano Halliburton
DVM, INTA, Argentina
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