|From:||=?UNKNOWN?Q?Agust=EDn?= Venzano |
Histonetters: I've lately been having some problems with blood films
staining. Despite previously filtering all reagents and strictly following
the required steps the results were frustrating.
Leukocytes' nuclei often appeared faintly stained, and their cytoplasms'
aspect was variable. Sometimes they looked well colored, but often
cytoplasmic coloration resulted dull. Moreover, eosinophils didn't show
there red cytoplasmic granules, instead their cytoplasm appeared rather
Of course I decided to immediately dispose of MG and G solutions in use, but
now I need a wise advise of you veteran netters: Many precious foul-stained
slides should be re-stained rather than disposed of. What should I do in
1. Decolor these slides.
2. Restain them with my new MG and G reagents?
Agustin Jose Venzano Halliburton
DVM, INTA, Argentina
<< Previous Message | Next Message >>