Re: puckers/snout
Trisha,
I like Louise Renton's suggestion, and would try that first. But there
is another neat little trick I use sometimes when I am having trouble
getting any section to lay flat and adhere properly; as soon as I
have picked the section up off the waterbath, I lay the slide (section
up) in between a filter paper (folded in half) and wetted with water,
and carefully roll the section flat using a glass specimen jar (the one
I use is similar to what you would use to store preserved parasite
samples in). Obviously take care to apply even pressure and be
careful not to twist the jar and cause damage to your section. I try
not to reuse the same place on filter paper from one section to the
next. (If I wasn't clear enough in my description, let me know and I'll
try again!) Good luck.
Greg
Date sent: Wed, 26 Sep 2001 15:39:17 -0700 (PDT)
From: "P. Emry"
Subject: puckers/snout
Forwarded to: DOBBIN@acad1.cs.upei.ca
To: HistoNet@pathology.swmed.edu
> I am cutting pig shouts...really I am. I am having problems with part of
> the internal structures lying nicely flat on the slide and others attached
> around it forming wrinckles and tiny folds.
>
> Any advice?
>
> Trisha
> U of Washington, Seattle
>
>
>
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Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada, C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
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