Rose, 
I use Quiagen protease at 1mg/ml in PBS for 10 minutes.  I do this
pre-treatment after my peroxidase quench and before the blocking step.
Jen



-----Original Message-----
From: Rose Richardson [mailto:rrichar3@iupui.edu]
Sent: Tuesday, September 18, 2001 12:30 PM
To: HistoNet Server
Subject: Protease pre-treatment


Does anyone have a protease pre-treatment method for use in IHC? 

Thanks!

Rose Richardson
Senior Research Techologist
Dr. Bernardino Ghetti's Lab
Ind.Univ.Med.Ctr./Pathology and Lab Medicine
635 Barnhill Drive, room A134
Indianapolis,IN 46202
Lab Phone: 317-274-1591


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