Re: unusual artefact

From:Cathy Locallo


Some time these strange patches can be due to not complete deparaffinization.
Change your xylenes more frequently and/or dry in the oven longer.

Cathy Locallo
Technical Assistance Center
Leica Microsystems

Aidan Schurr  on 09/04/2001 04:08:18 PM


Subject:  unusual artefact

hi all,

I have recently struck a strange staining artefact where the tissue has patches
where the haematoxylin doesn't seem to have been taken up.  The size seems to
vary from a dozen or so cells across to 10 or more times that size.  The eosin
staining appears to be normal in these areas.  Nothing should have changed in
our staining protocol, but I was wondering if anyone has any suggestions.

My thought so far are:  hot plates too hot, contaminated xylene, 95 and 100%
alcohols transposed, dud batch of haematoxylin (long shot).

Can anyone help?



aidan schurr
section head, histology
hutt valley district health board
lower hutt
new zealand
++64 4 570 9173

This message has been checked for all known viruses by the
MessageLabs Virus Scanning Service. For further information visit

<< Previous Message | Next Message >>