RE: unusual artefact
I would suggest making sure your xylene or xylene substitute is fresh.
Maybe the deparaffinization is not complete?
> -----Original Message-----
> From: Bill Sinai [SMTP:email@example.com]
> Sent: Tuesday, September 04, 2001 7:36 PM
> To: Aidan Schurr; firstname.lastname@example.org
> Subject: Re: unusual artefact
> Not so unusual artefact!
> This appears as a common artefact worldwide, we too have recently had this
> problem in several slides in the middle of a batch of H&E staining.
> It comes and goes, sometimes many slides and sometimes only a few. We
> not been able to relate it to any particular happening or condition or
> circumstance. We find it particularly noticable in cervical material
> although the last case was a colonic polyp.
> Good luck in finding an answer.
> Sorry not to be of help.
> Bill Sinai
> Laboratory Manager
> Tissue Pathology
> P.O. Box 533
> Wentworthville NSW 2145
> Subject: unusual artefact
> hi all,
> I have recently struck a strange staining artefact where the tissue has
> patches where the haematoxylin doesn't seem to have been taken up. The
> seems to vary from a dozen or so cells across to 10 or more times that
> The eosin staining appears to be normal in these areas. Nothing should
> changed in our staining protocol, but I was wondering if anyone has any
> My thought so far are: hot plates too hot, contaminated xylene, 95 and
> alcohols transposed, dud batch of haematoxylin (long shot).
> Can anyone help?
> aidan schurr b.m.l.sc
> section head, histology
> hutt valley district health board
> lower hutt
> new zealand
> ++64 4 570 9173
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