Maria,

We use a dilution of 1:400 (1 hr) on acetone-fixed frozen sections using a
LSAB immunoperoxidase technique.


Neil Hand,

Histopathology Dept,
Queen's Medical Centre,
Nottingham,
England UK



You wrote:

I need your help please.Is anyone using Novocastra's dysferlin
antibody(NCL-Hamlet)on muscle tissue?What dilution are you using and are you
using it on frozen or paraffin wax embedded tissue?

Any suggestions will be greatly appreciated.

Thanks

Maria


____________________________________________________________________
Neil Hand
Department Histopathology, University Hospital, Nottingham NG7 2UH.
work : Tel: (0115) 924 9924 extension 43725
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