Hi,
I have been asked to prepare and stain the grey matter of 4 mm slices of
brain for macroscopic study in a neuroscience course that will be taught
this semester. The method I have is a variation on a Mulligan stain that
was published by Roberts and Hanaway in Stain Technology 44(3). This
method has been used for the last thirty years with good results, but the
down side of the procedure is the production of strong vapors. The method
requires CuSO4-phenol mixture followed by quick wash in potassium
ferrocyanide. I have been told even with full safety measures taken (i.e..
use of gowns, respirators, double layer of gloves and the use of fume
hoods) it is still an unpleasant procedure. So, I was wondering does anyone
on this list have any experience staining whole brain slices and if they
do, can they suggest other methods that might not be as unpleasant. I look
forward to any suggestions you might have.
J. Foote
J. Foote
Histology Unit
Medical School Laboratories
Faculty of Medicine
Memorial University of Newfoundland
St. John's, Newfoundland
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