Sectioning fatty tissues

From:a i d a n s c h u r r <>

Hi all,

We have a problem, where our routine processing regime is not 
adequate for very fatty tissues, such as breast or lipoma.  
Basically, blocks are not cuttable - we simply get a thin ring of 
tissue with a hole in it.  The block itself seems slightly spongy.  
My guess is that the lipid is preventing adequate infiltration of the 
paraffin.  The question:  Does anyone out there use a delipidising 
agent to treat these kind of tissues, and if so, what do you use.  Or 
am I on the wrong track?!

Thanks in advance,
Aidan, New Zealand.

shin: device for finding furniture in the dark...
a i d a n   c   s c h u r r 
     section head,  histology department
      hutt valley health
       high street
        lower hutt, new zealand

     ph.  ++64 4 5709173
     fax  ++64 4 5709214

<< Previous Message | Next Message >>