Sectioning fatty tissues
|From:||a i d a n s c h u r r <Aidan.Schurr@hvh.co.nz>|
We have a problem, where our routine processing regime is not
adequate for very fatty tissues, such as breast or lipoma.
Basically, blocks are not cuttable - we simply get a thin ring of
tissue with a hole in it. The block itself seems slightly spongy.
My guess is that the lipid is preventing adequate infiltration of the
paraffin. The question: Does anyone out there use a delipidising
agent to treat these kind of tissues, and if so, what do you use. Or
am I on the wrong track?!
Thanks in advance,
Aidan, New Zealand.
shin: device for finding furniture in the dark...
a i d a n c s c h u r r
section head, histology department
hutt valley health
lower hutt, new zealand
ph. ++64 4 5709173
fax ++64 4 5709214
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