Re: Nerve Growth Factor Receptor
|From:||"L. Gibbs" <email@example.com>|
I have used P75 antibodies frequently over the past 15
years. (One of our researchers prepared the original clone for
I usually use monoclonal but this applies to polyclonals as well:
P75 results are excellent with methcarn/paraffin embedded
sections. If you must use formalin-fixed specimens, you should
still get good results with pronase or the other antigen
retrieval methods. I have used various P75 antibodies in the
1-5ug/ml range, usually overnight at rt or 2hrs rt, followed by
overnight at 4C.
Physiology & Biophysics
On Mon, 25 Sep 2000, Philopena, Jennifer wrote:
> This past NSH conference was my very first. I learned so so much, enjoyed
> all of the workshops that I attended, and it was great to finally match so
> many names with faces. And the freebies at the vendor show weren't bad
> either. I was very surprised, however, that there were so few posters. But
> thank you all for making it so worth the trip.
> Back to work...
> Have any of you labeled Nerve Growth Factor Receptor p75? I'm using a
> polyclonal from Chemicon, and I've tried proteinase and HIER pre-treatments
> in FFPR tissue. Thanks for any tips. Jen
> Jennifer M. Philopena
> Scientist 1
> Canji, Inc.
> 3525 John Hopkins Court
> San Diego, CA 92121
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