Re: pcna

From:HOOVER_JENNIFER@LILLY.COM (by way of histonet)

     Hi Robin!  When I had to stain mouse tissue in the past for PCNA I
used a PCNA kit from Zymed. All reagents are ready-to-use.  The primary
antibody is a biotinylated PCNA, thus eliminating the mouse on mouse
non-specific/background staining issues.  Although I do believe the clone
is PC10.  I did have to tweak the protocol a bit to suit my particular
situation. I think that I reduced the incubation time in the
streptavidin-peroxidase conjugate. Sometimes I have also found it helpful
to incubate the primary and secondary antibodies at 4C to also reduce
non-specific/background. Normally I am not a big fan of ready made
solutions but this kit was fantastic for my purposes.  I hope this
information is helpful. Please do not hesitate to contact me if you have
further questions. Best wishes!

Jennifer Hoover
Eli Lilly and Company

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