Re: Internal Elastic Lamina

From:Geoff McAuliffe <mcauliff@UMDNJ.EDU> (by way of histonet)

Taffy wrote:

> Hello AGAIN Histonetters,
> I have a big problem..PLEASE help, if you can..
> I have an investigator that is looking for a stain for the internal Elastic
> Lamina (IEL) in THICK (70um) plastic sections (yep, this is still the
> artery)..they are wanting something that will stain this so they can do
> histomorphometry..ANY ideas?????????
> Thanks again for your help!!!
>
> Taffy Hooser
> **************************************
> Mary (Taffy) Hooser, HT(ASCP)
> Department of Anatomy and Cell Biology
> Indiana University School of Medicine
> MS-5035, 635 Barnhill Drive
> Indianapolis, IN. 46202-5120
> Tel: (317)274-2308.
> Fax: (317)278-2040.
> E-mail: hooser@anatomy.iupui.edu
> ***************************************

"A routine method for contrasting elastin at the ultrastructural level". Franc,
S. et al. J. Histochem. Cytochem. 32:251-258, 1984.
They used uranyl acetate in methanol.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************




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