Re: MEASURING ACCURATELY

From:Geoff McAuliffe <mcauliff@UMDNJ.EDU> (by way of histonet)

Louise Taylor wrote:

> Hello histonetters
>
> I want to ask a REALLY DUMB question: How does one accurately measure out
> Tween 20 ( pipette, measuring cylinder)? It is so  viscous that I always
> doubt that i have washed out/expelled the correct amount.
>

    I put 90 ml of dist. water in a 100 ml graduate, then carefully add
Trition or Tween to the 100 ml mark. Drop in a small stir bar, put it on the
mag. stirrer for a while, and you have a relatively easy to handle 10%
solution.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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