Re: Immuno after H&E on a in-situ slide
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From: | Dana Settembre <settembr@UMDNJ.EDU> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Date: | Mon, 31 Jan 2000 22:47:18 -0500 |
Content-Type: | text/plain; charset="us-ascii" |
On Wed, 27 Oct 1999, Cynthia A Delong wrote:
>
>
> Hello everyone,
>
> I have a question regarding doing immuno on a in-situ after it has been
> stained with H & E. Does anyone have any sort of protocol for this.
>
> I believe after removing the coverslip I would run it backwards to water and
> then start my procedure right???? Anyway if anyone has such a thing
>would you
> please forward it to me. Do you do antigen retrieval on this section. What
> damage if any will the in-situ have. Note: It has been labeled with a
> radioactive probe.
>
> Thanks for all of your help
> Cindy
> 317-276-7635
> Fax: 317-277-6146
>
>
>
Hello Cynthia,
My experiences have brought me a bit into the direction of what your
asking but not exactly.
I have run immuno on slides that have been stained with H & E. They work
just fine. Just as you said, you work the coverslip off and run them back
into water and start your procedure in some instances I retrieve and in
other instance I have not. Both work fine. Hope this helps even though
it's not exactly what your looking for.
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark, New Jersey
USA
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