Re: Immuno after H&E on a in-situ slide

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From:Dana Settembre <settembr@UMDNJ.EDU> (by way of histonet)
Date:Mon, 31 Jan 2000 22:47:18 -0500
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On Wed, 27 Oct 1999, Cynthia A Delong wrote:

> Hello everyone,
> I have a question regarding doing  immuno on a in-situ after it has been
> stained with H & E.   Does anyone have any sort of protocol for this.
> I believe after removing the coverslip I would run it backwards to water and
> then start my procedure right????  Anyway if anyone has such a thing
>would you
> please forward it to me.   Do you do antigen retrieval on this section.  What
> damage if any will the in-situ have.  Note: It has been labeled with a
> radioactive probe.
> Thanks for all of your help
> Cindy
> 317-276-7635
> Fax:  317-277-6146
Hello Cynthia,
My experiences have brought me a bit into the direction of what your
asking but not exactly.
I have run immuno on slides that have been stained with H & E. They work
just fine.  Just as you said, you work the coverslip off and run them back
into water and start your procedure in some instances I retrieve and in
other instance I have not.  Both work fine. Hope this helps even though
it's not exactly what your looking for.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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