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From:LuAnn Anderson <> (by way of histonet)
Date:Mon, 31 Jan 2000 22:47:18 -0500
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From: "LuAnn Anderson"  <ander093>
Date: Thu, Oct 28, 1999 10:03 AM
Subject: Re: warm water block soak

Responding to the message of <>

I have been a certified histotech for 18 years.  When I went through my
training, we were taught to soak briitle blocks, after facing, in warm water
with a drop of liquid dish soap.  I have used this method routinely and it
quite well.  It works very well for sectioning of large blocks of brain tissue
also.  Face the blocks to a full section, place face down in a shallow dish of
warm water containing one-two drops of liquid dish soap.  Just prior to
sectioning, place the blocks on ice to cool.  When all else fails, try this.
For extremely hard or brittle blocks, use warm water with a few drops of
Ammonium  Hydroxide and soak in the same manner.  I must admit, the fabric
softener suggestion is intriguing!
>LuAnn Anderson,HT
Neuropathology Lab-University of Minnesota

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