RE: warm water block soak
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From: | "O'Brien, Sue" <histo@bthosp.com> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Date: | Mon, 31 Jan 2000 22:47:22 -0500 |
Content-Type: | text/plain; charset="us-ascii" |
What an interesting thought - to do it with WARM water. I have been placing
pre-trimmed blocks onto ice that has paper toweling on top and is moistened
(but not flooded!) for years. By the time I get back to my first block, it
has been adequately soaked and I have plenty of sections that are usable
before the tissue starts drying out. I happen to prefer this method because
it saves on knife blade usage and chills the block at the same time that it
is soaking it. (Do you have a problem with compression, or do you chill your
blocks AFTER the warm soak?) Regards,
Sue O'Brien
Histology Supervisor
Burdette Tomlin Memorial Hospital
Cape May Court House, NJ 08210
e-mail: histo@bthosp.com
-----Original Message-----
From: NEVADUNNE@aol.com [SMTP:NEVADUNNE@aol.com]
Sent: Wednesday, October 27, 1999 8:16 PM
To: histonet@pathology.swmed.edu
Subject: warm water block soak
Hi everyone,
I'm an old war-horse HT working in a small community hospital
doing
routine histology. I'm just curious if anyone else has used a warm
water soak
for their paraffin blocks (after facing) to improve sectioning. I
have found
this technique very helpful in the sectioning of brittle biopsies
and bloody
specimens. The crushed ice and water soak prior to sectioning allows
a few
good sections after soak, but the warm water soak lets us get
several ribbons
before we encounter dried out tissue. This has cut down on chatter,
time
spent re-soaking and over-all quality of sections.
Any feedback?
Maureen Tomblin HT(ASCP)
Union Hospital
Elkton, MD
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