RE: warm water block soak

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From:"Weems, Joyce" <JWEEMS@sjha.org> (by way of histonet)
To:histonet@histosearch.com
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Date:Mon, 31 Jan 2000 22:47:22 -0500
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What kind of paraffin are you using? Does it melt any?
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta


	-----Original Message-----
	From:	NEVADUNNE@aol.com [SMTP:NEVADUNNE@aol.com]
	Sent:	Wednesday, October 27, 1999 8:16 PM
	To:	histonet@pathology.swmed.edu
	Subject:	warm water block soak

	Hi everyone,
	    I'm an old war-horse HT working in a small community hospital
doing
	routine histology. I'm just curious if anyone else has used a warm
water soak
	for their paraffin blocks (after facing) to improve sectioning. I
have found
	this technique very helpful in the sectioning of brittle biopsies
and bloody
	specimens. The crushed ice and water soak prior to sectioning allows
a few
	good sections after soak, but the warm water soak lets us get
several ribbons
	 before we encounter dried out tissue. This has cut down on chatter,
time
	spent re-soaking and over-all quality of sections.
	Any feedback?

	Maureen Tomblin HT(ASCP)
	Union Hospital
	Elkton, MD




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