Greg Tesdall, Alcohol/Xylene Processing

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From:BB racing <bbracing@silk.net> (by way of histonet)
To:histonet@histosearch.com
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Date:Mon, 31 Jan 2000 22:47:31 -0500
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Greg,
           Nice to hear from you, and that you are still happy with the
alcohol/xylene processing method.  Also nice to hear that you have been
able to use one to the xylene substitutes successfully.  I have several
gallons of various substitutes sitting around my lab, but have never had
the time to try any of them out, so now I don't need to.  Also nice to hear
that you have encountered some technologists who are interested in trying
out the technique.  There are no changes to the basic method, so feel free
to disseminate the information to anyone who is interested.  Since I
presented this paper at the NSH convention some years ago, I have tried to
have it published, but it has always been rejected for a whole host of
reasons, ( "goes against generally accepted histological principals,"
"can't possibly work, water and xylene are not missible," "it would be to
harsh for small delicate biopsies," " fixation/dehydration/clearing is
excessive," " would over harden tissues," "sectioning would be impossible,"
"would destroy tissue antigens," " too many non factual statements,"
"flawed reasoning," " too long" "insufficent references," "too informal a
writing style,") ect, ect, so the manuscript sits in a box in my basement,
but one day....
Kerry Beebe
Kelowna Gen Hospital
Kelowna B.C. Canada.
bbracing@silk.net




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