Re: Saponin reference(s)

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From:"J. A. Kiernan" <jkiernan@pony.its.uwo.CA>
To:"Bourassa, Patricia" <>, "Histonet @Pathology.Swmed.Edu (E-mail)" <>
Date:Mon, 11 Oct 1999 23:18:01 -0400 (EDT)
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At 09:57 AM 10/11/99 -0400, Bourassa, Patricia wrote:
>Hi, all!
>I can't seem to find my paper on use of Saponin in IHC.  Does anyone out
>there have a good reference they use?  I'm hoping it matches mine!

 Here are two. The saponin permeabilization occurs during
 fixation with these methods. The second paper is on use of
 the method for EM immunohistochemistry.

Pignal, F., Maurice, M. and Feldmann, G. 1982. Immunoperoxidase localization of albumin and fibrinogen in rat liver fixed by perfusion or immersion: effect of saponin on the intracellular penetration of labeled antibodies. J.  Histochem. Cytochem. 30: 1004-1014.

Willingham, M. C. 1983. An alternative fixation-processing method for  preembedding ultrastructural immunocytochemistry of cytoplasmic antigens: the GBS (glutaraldehyde-borohydride-saponin) procedure. J. Histochem. Cytochem. 31: 791-798.
                                                           John Kiernan
                                                            London, Canada.

                    John A. Kiernan
                    Dept of Anatomy and Cell Biology
                    University of Western Ontario
                    LONDON,   Canada.

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