Re: DiI and plastic embedding

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From:"J. A. Kiernan" <jkiernan@pony.its.uwo.CA>
To:"German Pinzon-Duarte" <>, <>
Date:Mon, 11 Oct 1999 23:18:04 -0400 (EDT)
Content-Type:text/plain; charset="iso-8859-1"

At 09:44 AM 10/11/99 +0200, German Pinzon-Duarte wrote:

>I would like to use DiI-labeling together with a plastic embedding.
>Does anyone have experience with this? The protocol requires alcohol and Im not sure wheter DiI fades with alcohol or not.

  DiI is soluble in alcohol and other organic solvents. It should
  be OK for gelatin embedding if the tissue needs more support,
  but you will then have to cut frozen sections. Possibly one of
  the water-soluble (PEG) waxes would be OK. They are troublesome
  to cut for people who do not work with PEG all the time.

                    John A. Kiernan
                    Dept of Anatomy and Cell Biology
                    University of Western Ontario
                    LONDON,   Canada.

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