I will be receiving breast tissue which has been snap frozen and maintained
at -70C.  We would like to thaw the tissue and divide it for use in RNA
extraction and immunohistochemistry.  The tissue for IHC will be
subsequently fixed in 10% NBF and paraffin embedded.

I need some suggestions for how to thaw the tissue and subsequently fix it
in order to do the least possible damage to it physically and chemically.

Thanks in advance for any help you can give.

Mary Ross
Med Micro and Immuno
Ohio State University