Dave Tacha wrote:
>
> Thanks for everyones response for CD4.  I forgot to add CD8. Is there a
> CD8 that works in formalin-fixed paraffin-embedded tissues?  Is there
> any special pretreatment?

Yes, we use DAKO's in citrate buffer either with one minute pressure
cooker, or microwave 7 min or at 94 degree heat-treatment.  We used it
at 1:50 dilution manually or 1:100 dilution in Ventana's Nexus
autostainer.

Hope this help.