Re: CD8 for paraffin-embedded tissues

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From:Wangxue Chen <> (by way of histonet)
To:histonet <>
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Dave Tacha wrote:
> Thanks for everyones response for CD4.  I forgot to add CD8. Is there a
> CD8 that works in formalin-fixed paraffin-embedded tissues?  Is there
> any special pretreatment?

Yes, we use DAKO's in citrate buffer either with one minute pressure
cooker, or microwave 7 min or at 94 degree heat-treatment.  We used it
at 1:50 dilution manually or 1:100 dilution in Ventana's Nexus

Hope this help.

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