Re: old slides
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From: | Yvan Lindekens <yvan.lindekens@skynet.be> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
In my opinion the best way to remove coverslips from old slides is by
soaking in xylene or toluene untill the coverslip comes off without
"help"...
This can take a few days, but the process can be speeded up by soaking in
xylene or toluene in a tightly closed jar at about 40°C...
Long storage in xylene, even when slightly acidic, isn't a problem as the
colorability of the sections can usualy be restored by soaking in:
* hydrogen peroxide 3% for 30 min, or, better
* 10% solution of benzoylperoxide in aceton for 15 min.
I didn't have much luck with heating slides over a bunsenburner and
"sliding off" coverslips...
Celloidizing the sections prior to restaining is a good idea...
Fading of H&E staining can be (more or less) prevented by
* decent washing after staining
* "bluing" in a solution of known composition
* second "bluing" step in ammonia vapour for a few seconds, the H stain
turns into a nice dark blue
* dehydration in solutions of good quality
* the use of good (neutral) mountant
* good storage conditions of the slides
The use of "slightly alkaline mountants" is a good idea, but keep in mind
that some other stains tend to fade very fast in alkaline mountants
(malachite green oxalate, acid fuchsin...).
Yvan Lindekens.
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