Cartilage Decalcification

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From:Pierre Mainil <> (by way of histonet)
To:histonet <>
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Hello histonetters,

Another question to cartilage lovers.
What is the best decalcification system.
We observe important variability with Saf O staining although using
Has some one ever used EDTA with etylpyrimidium  during the
decalcfication process.
Advice and help appreciated.

Pierre Bern

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