John & Tracey MacKinnon wrote:
>
>  Has any one ever encountered nuclear bubbling when using a microwave
>pressure cooker for HIER?  Have I just overcooked them??
>
> John

Is it just SOME tissues, SOMETIMES? Particularly NBF fixed?

If so, it's possible that those tissues were not completely
fixed. The old "minimum of 6-8-12 hours to overnight" fixation
in NBF is a thing of the past. We see the nuclear bubbling when
"someone" increases the temp on the slide dryer after sectioning,
particularly on those tissues that arrive late in the day. Those
sections fixed in mercury (don't yell at me) or fixed all day
in NBF do not seem to have this problem as often.

It's a thought.
--
Peggy A. Wenk, HTL (ASCP)
Anatomic Pathology
Wm. Beaumont Hospital
3601 W. 13 Mile Rd.
Royal Oak, MI 48073-6769