Mary, Nora et al.
KPL (Kirkegaard & Perry Laboratories) puts out a useful manual for
peroxidase/alk. phos. double staining, complete with protocols.  Vector
Laboratories also has double staining procedures available. I have  found
this article to be useful; Practical suggestions for successful immunoenzyme
double-staining experiments. Van der Loos et al.,Histochemical J,25:1-13,
1993.

Brett

Brett M. Connolly, Ph.D.
Merck Research Laboratories
WP26A-3000
P.O. Box 4
West Point, PA 19486
ph.215-652-2501
FAX. 215-652-2075
email:brett_connolly@merck.com

> ----------
> From: 	Nora Fox
> Sent: 	Tuesday, October 27, 1998 3:47 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	Re: double labeling
>
> I would like to see this too--as may lots of others.
>
> Thank you to all of you who have responded to my many questions.
>
> Nora
>
> >
> >>Good Morning,
> >>        Would someone who has some experience with double labeling[IHC]
> >>please e-mail me directly? I have a few questions from one of my
> >>colleagues...we're interested in one Ab w/DAB and one with alk.phos.
> Thanks
> >>				Best Regards,
> >>				Mary Vaughan HT(ASCP)
> >>				Roswell Park Cancer Institute
> >>				Pharmacology + Therapeutics
> >>				Elm + Carlton Sts.  CDC-121
> >>				Buffalo, NY 14263
> >
> >
> >
> Nora Fox
> Division of Orthopaedic Surgery
> Stanford University
> Edwards R144, MC 5341
> 300 Pasteur Drive
> Stanford, CA 94305
> phone: (650)725-2746
> fax:  (650)723-6396
>