Re: Safranin O / Fast Green

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From:John Difford <> (by way of histonet)
To:histonet <>
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Re: Safranin O / Fast Green Method

Attn.Dr Pierre Mainil-Varlet

Dear Pierre,

The Safranin O method for Cartilage goes like this;

1. Dewax section and take to water
2. Stain nuclei with a suitable iron haematoxylin
3. Blue in running tapwater
4. Rinse in distilled water
5. Stain with 1% light green diluted 1 in 5 with distilled water, for 3
6. Rinse in 1% acetic acid
7. Stain with 0.1% Saffranin O, for 4 - 6 minutes
8. Rinse in 1% acetic acid and check under microscope. Any overstaining
with Safranin can be modified by re-applying the light green     solution
briefly, and vice versa.
9. Dehydrate with alcohol,clear and mount

Modified from the method in Lillie 'Histopathological Technic and Practical


John Difford FIBMS
Histopathology Dept
Royal Free Hospital
London, England.

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