Icam-1 in Rat brains paraffin

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From:Jamie Erickson <JErickson@genetics.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Hi all,
        Is anyone using the Pharmingens I-CAM-1
CD54 antibody? I've tried it in these rat brains
(Paraffin) I have But had no luck with trypsin
digestion @ 20 minutes @ 37. I'm wondering
what digestion to use? Any Ideas?

Jamie Erickson
Genetics Institute

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