frozen sections

<< Previous Message | Next Message >>
From:aries@a.imap.itd.umich.edu (Patricia Landry) (by way of histonet)
To:histonet <histonet@magicnet.net>
Reply-To:
Content-Type:text/plain; charset="us-ascii"

Hello,  I have questions concerning a new project I am starting.  It
involves human arteries.  The tissue is already frozen but not fixed when I
receive it.  My job is to cut cross sections and perform basic H&E and
immunofluorescence. I have previously only worked with decalcified fixed
tissue which I embed in O.C.T.  My questions are:  How do I embed it or do
I need to embed in order to cut it? What temperature? How thick?  Also, do
I need to fix on the slide and with what fixative? I understand that some
fixatives might inhibit my antigen detection?  All the references I can
find so far refer to cultures or smears, not frozen sections on slides.  I
would appreciate any help or advise.
Pat Landry




<< Previous Message | Next Message >>