Cartilage Decalcification and embedding

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From:Pierre Mainil <> (by way of histonet)
To:histonet <>
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Dear all,

I want to thank everyone who responded to my question.  I am sure some
tricks will work. I will start to try with the positively charged
Concerning the soakink I'm little bit affraid about possible interaction
with immunohistochemistry.
Does some once has a detail protocol for SAF O/Fast Green ?
Does some known where anti col X (human) are available from?
Once again, thank you all for your generous responses!

Pierre Mainil-Varlet, MD, PhD
Dept of pathology
University of Bern

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