Beverly,
I use Biogenex prediluted super sensitive IA4 with no antigen retrieval and
a 30 min room temp incubation.  Works very nicely on the myofibroblastic
lesions I'm interested in and vessel myopericytes always stain as a
positive control.
Jeff SIlverman
peptolab@hamptons.com

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From: beverly.miller@shandon.com
To: histonet@pathology.swmed.edu
Subject: HELP: smooth muscle actin antibody
Date: Thursday, October 08, 1998 12:26 PM

Looking for experts on the use of smooth muscle actin antibody, in
particular clone 1A4, on small vessels in lung tissue, especially those who
have done morphometric analysis of smooth muscle cells. Help with
protocols, fixation issues, epitope retrieval, etc.

Thanks
Bev Miller
Shandon Lipshaw
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