Re: Factor X111a Protocol

<< Previous Message | Next Message >>
From:Jeff Silverman <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

I agree essentially agree with Bryan about the factor XIIIa protocol.  I've
been obsessed with FXIIIa+ histiocytic  cells for four years and have
written about it a few times.  I use the Calbiochem rabbit annti-human
diluted 1:400 in PBS with 1% bovine serum albumen added.  I digest formalin
fixed tissues for 30 min at 37 degrees using the Sigma trypsin tablets and
the antibody incubation I use is 30 minutes at room temp.Sometimes omitting
digestion works but I've had more consistent results by using the trypsin.
A few tumor cases I've studied that were fixed in HistoChoice wouldn't
stain after digestion but were fine without it. Hope this helps.
Jeff Silverman
Southampton NY USA Hospital (for now)

> From: Dave Tacha <>
> To:
> Subject: Factor X111a Protocol
> Date: Thursday, October 08, 1998 11:30 AM
> We've been using a polyclonal antibody to the A-subunit of human
> coagulation Factor XIII (Factor XIIIa).  It seems to work fine without
> any pretreatment.  We used skin, placenta and a capillary hemangioma for
> controls.  We tried digestion and it didn't seem to make much
> difference.  However, the literature shows many procedures using either
> trypsin or antigen retrieval methods.  Could someone who has work a lot
> with this antibody please enlighten me?
> Thanks

<< Previous Message | Next Message >>