Can you post a pic of this non specific staining? What organ is it?
Is it inside blood vessels?
At 5:27 PM -0500 10/7/08, Jan Shivers wrote:
>Addendum... I should have included this information in my original posting...
>I'm already doing a 3% H2O2 peroxidase quench (10-15 minutes) and a
>10% normal serum protein block prior to primary Ab, and a 2-4% case
>species serum addition to the link Ab.
>Is there something ELSE that I could use to block binding to
>platelets (or what appears to be platelets, though it could be bits
>of proteinaceous cellular debris that's binding Ab nonspecifically)?
>----- Original Message -----
>From: Jan Shivers
>Sent: Tuesday, October 07, 2008 3:14 PM
>Subject: platelets and IHC
>Has anyone ever encountered platelets staining nonspecifically when
>using an antibody directed against other antigens? If so, how do
>you quench that activity? Thanks.
>Histology/IHC/EM Section Head
>University of Minnesota
>Veterinary Diagnostic Laboratory
>1333 Gortner Ave.
>St. Paul, MN 55108
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