True, but unless you have every slide on the run as titration the machine
wont start.  That's a good trick if you just have a titration run.

Mark
On Tue, Oct 14, 2008 at 11:37 AM, Sebree Linda A. wrote:

> Yes, you can always run in "manual" mode....editing the protocol to
> "titration" rather than "antibody".  That does indeed allow you to
> manually apply the antibody when the instrument signals its time to do
> so.
>
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Milne,
> Katy
> Sent: Tuesday, October 14, 2008 12:45 PM
> To: arsenn@hsh.org; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Expiration dates
>
> Hi Amy,
>
> I use the Ventana Discovery and I add my antibodies manually as I'm in
> research and don't feel like shelling out extra for empty containers to
> put my Abs in.  Do you have an option on the Benchmark to switch the
> primary Ab step to "titration" or something like that (can't remember
> what the exact step name is but not the autodispense one).  I don't even
> know if that's an option on the Benchmark but with the Discovery, I can
> just open that machine and add it straight at that step.  Perhaps you
> could get your antibody out of the dispensor and try it that way.
>
> Katy Milne
> Deeley Research Centre
>
> -
> Message: 4
> Date: Mon, 13 Oct 2008 07:04:10 -0400
> From: "Senn, Amy R" 
> Subject: [Histonet] Expiration dates
> To: 
> Message-ID:
>       
> Content-Type: text/plain;       charset="us-ascii"
>
> Hello all,
>
>
>
> My supervisor wanted me to ask everyone who's posting about the
> expiration dates of antibodies:  How can you use expired antibodies on a
> stainer such as a Benchmark-which will not allow you to start the run
> due to expiration dates on the containers?
>
>
>
>
>
> Thanks!!
>
>
>
> Amy Senn
>
> Camp Hill, PA
>
>
>
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