RE: [Histonet] Expiration dates

From:"Sebree Linda A."

Yes, you can always run in "manual" mode....editing the protocol to
"titration" rather than "antibody".  That does indeed allow you to
manually apply the antibody when the instrument signals its time to do

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792

-----Original Message-----
[] On Behalf Of Milne,
Sent: Tuesday, October 14, 2008 12:45 PM
Subject: RE: [Histonet] Expiration dates

Hi Amy, 

I use the Ventana Discovery and I add my antibodies manually as I'm in
research and don't feel like shelling out extra for empty containers to
put my Abs in.  Do you have an option on the Benchmark to switch the
primary Ab step to "titration" or something like that (can't remember
what the exact step name is but not the autodispense one).  I don't even
know if that's an option on the Benchmark but with the Discovery, I can
just open that machine and add it straight at that step.  Perhaps you
could get your antibody out of the dispensor and try it that way.

Katy Milne
Deeley Research Centre 

Message: 4
Date: Mon, 13 Oct 2008 07:04:10 -0400
From: "Senn, Amy R" 
Subject: [Histonet] Expiration dates
Content-Type: text/plain;       charset="us-ascii"

Hello all,

My supervisor wanted me to ask everyone who's posting about the
expiration dates of antibodies:  How can you use expired antibodies on a
stainer such as a Benchmark-which will not allow you to start the run
due to expiration dates on the containers?


Amy Senn

Camp Hill, PA

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