Re: [Histonet] Dissolve plastic

From:Joseph Saby

Lee and Peggy-We section these eyes all the time with the plastic in place.  I would certainly suggest a gluaraldehyde fixative.  Straight NBF is a very poor fixative for the many tissues found in eyes, especially the retina.  If you contact me directly, we can talk off line.  Eyes have held a special fascination with me for many years.Joe SabyNAMSA________________________________From: Lee & Peggy Wenk To: histonet@lists.utsouthwestern.eduSent: Monday, November 24, 2008 12:47:24 PMSubject: [Histonet] Dissolve plastic=0AI Need Histonetters help.We have an eye with a plastic part for the lens, which is a type oftelescope, I've been told.According to the new pathologist, we need to dissolve out this plastic lens,then he can gross the eye and we can process it into paraffin.We've never had to dissolve plastic before. The eye is in 10% NBF right now.I'm guess maybe something like chloroform? But what percent? For how long?Before or after grossing?Has anyone done this before, that can send a procedure to me? Thanks.***************************************=0APeggy A. Wenk, HTL(ASCP)SLSProgram Director,  Schools of HistotechnologySupervisor, Mortuary ServicesLaboratory Safety Officer, Disaster Preparedness and Quality AssuranceCoordinatorDepartment of Anatomic Pathology, 100ROWilliam Beaumont Hospital3601 W. 13 Mile RoadRoyal Oak, MI 48073-6769Work  248/898-9079Fax      248/898-9054E-mail  pwenk@beaumont.eduWeb Page: _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu      
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