Re: [Histonet] Buffer.

From:Tora Bardal

Hello Ian,

We use a mixture of 2.5% glutardialdehyde, 2.5% paraformaldehyde and 
0.5% sucrose in 0.08 M cacodylic buff€er, pH 7.4 to 7.6

Galloway, Trina Falck; Kjørsvik, Elin; Kryvi, Harald.
Effect of temperature on viability and axial muscle development in 
embryos and yolk sac larvae of the Northeast Arctic cod (*Gadus 
morhua*).. /Marine Biology/ 1998 ;Volum 132.(4) s. 559-567


NTNU Center of Fisheries and Aquaculture

Ian Montgomery wrote:

>            What is the recommended EM buffer for sea water fish? I had
>thought of using sea water itself but would cacodylate or one of the
>phosphates be better and at what molarity? 

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