[Histonet] Reprocessing Hematoxylin&Eosin tongue slides.

From:"Pumla Pamla-Gutter"


Could anyone help me with a protocol for re staining H&E formalin-fixed
tissue sections?

I have some H&E stained tongue slides that are too weak and would like to
reprocess them.

Could I just soak them in xylene, rehydrate and then place in hematoxylin?
Any ideas what that does to the counterstain with Eosin?

                 By the way: I used Gill's Hematoxylin (water+ethylene
gycol+hematoxylin+sodium iodate+aluminum sulfate+acetic acid)

Thank you,

Pumla Pamla-Gutter

Research Assistant I

Ohio State University
College of Dentistry
305W. 12th Avenue
Columbus, OH, 43232

Office: (614) 292-4046
Fax: (614) 247-6945
Email: pamla-gutter.1@osu.edu
Histonet mailing list

<< Previous Message | Next Message >>