RE: [Histonet] Reprocessing Hematoxylin&Eosin tongue slides.

From:"Mickie Johnson"


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Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
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Spokane, WA 99203
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-----Original Message-----
[] On Behalf Of Pumla
Sent: Thursday, November 13, 2008 11:09 AM
Subject: [Histonet] Reprocessing Hematoxylin&Eosin tongue slides.


Could anyone help me with a protocol for re staining H&E formalin-fixed
tissue sections?

I have some H&E stained tongue slides that are too weak and would like to
reprocess them.

Could I just soak them in xylene, rehydrate and then place in hematoxylin?
Any ideas what that does to the counterstain with Eosin?

                 By the way: I used Gill's Hematoxylin (water+ethylene
gycol+hematoxylin+sodium iodate+aluminum sulfate+acetic acid)

Thank you,

Pumla Pamla-Gutter

Research Assistant I

Ohio State University
College of Dentistry
305W. 12th Avenue
Columbus, OH, 43232

Office: (614) 292-4046
Fax: (614) 247-6945
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