RE: [Histonet] GI Biopsies

From:"Mickie Johnson"

Dear Andria,

This sounds like over-processing. If you are using a regular processing
schedule it is for sure. At Sacred Heart Medical Center in Spokane we used a
2 hour 45 minute program with 15 minutes in each station. We still soaked
the blocks in water before icing and cutting. Sometimes we had to soak for
the second or third level. We fixed in Bouin's. Also cutting with a slower
speed as the knife passed through the tissue helped. Hope this helps

Best Regards,
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
  & Mohs Suite Mohs Reporting Software
2507 S. Manito Blvd.
Spokane, WA 99203
FAX   509-624-3926
Web: & &
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-----Original Message-----
[] On Behalf Of Evans,
Andria B.
Sent: Thursday, November 13, 2008 12:46 PM
Subject: [Histonet] GI Biopsies

We are having difficulty with our GI biopsies having a lot of chatter on the
H & E.  I think somehow they are getting over processed, but not sure....
has anyone had this problem or could suggest what might be causing this
and/or solutions.  
Thank you!
Andria B Evans, HTL(ASCP)CM
Anatomic Pathology
York Hospital
1001 S. George Street
York, PA  17405
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