----- Original Message ----- 
From: "Bryan Llewellyn" 
To: "McKnight, Tanisha" 
Sent: Friday, October 24, 2008 9:34 AM
Subject: Re: [Histonet] PAS with Glycogen Digestion - Solution Not Working


> Try porcine pancreatic alpha amylase from Sigma (A 3176).  I used the 
> amount to cover a dime 1 mm deep in 15 mL distilled water and it always 
> worked for me in an hour at room temperature.
>
> However, in general, you should base your concentration on the amount of 
> enzyme you need rather than the powder that contains the enzyme.  That is 
> 0.5 grams of a 10 units per gram powder is not the same concentration as 
> 0.5 grams of a 100 units per gram powder.
>
> Bryan Llewellyn
>
> ----- Original Message ----- 
> From: "McKnight, Tanisha" 
> To: 
> Sent: Friday, October 24, 2008 8:26 AM
> Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working
>
>
>> Hi All:
>>
>> I am in the process of working up a PAS Staining protocol. The PAS stain
>> is working fine, but I am unable to get my tissue to digest. I am using
>> .5g of Diastase in 50ml of DI water. I tried heating the solution in a
>> water bath for 30 min, 1 hour and 1.5 hours and it still looks the same
>> as the one without digestion. Could I be using the wrong Diastase? If
>> so, does anyone have any suggestions for a new product?
>>
>> Thanks,
>>
>> Tanisha Neely, HT(ASCP)
>> AP-Histology/Specimen Management
>> 
>>
>>
>>
>>
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>
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