Anna,It has been my experience that most tissues are OK if soaked for a long time; but some tissues (especially fatty ones, like brain) will swell (a lot!)  when soaked for an extended period of time.Just be careful!Kim Kim Merriam, MA, HT(ASCP)Cambridge, MA----- Original Message ----From: Anna K. Schultz To: histonet@lists.utsouthwestern.eduSent: Wednesday, October 24, 2007 11:05:37 AMSubject: [Histonet] curious about soaking paraffin blocks.Greetings All,  I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this?   I am just plain curious and still fairly new to the field.  I often leave blocks on for a long time and this seems to help or at least not hurt.Thanks,Anna-- Anna K. SchultzSDRC Core A Research TechnicianDepartment of DermatologyCollege of Physicians and SurgeonsColumbia UniversityVanderbilt Clinic 15-211630 West 168^th StreetNew York, NY 10032Phone: (212) 305-4954_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet__________________________________________________Do You Yahoo!?=0ATired of spam?  Yahoo! Mail has the best spam protection around http://mail.yahoo.com 
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