We judge the amount of time on the ice by the tissue.  The paraffin
never comes into play.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K.
Schultz
Sent: Wednesday, October 24, 2007 11:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] curious about soaking paraffin blocks.

Greetings All,
  I remember reading on here/archives that paraffin blocks should not be

soaked or kept on ice for too long--what is the reasoning behind this?  
I am just plain curious and still fairly new to the field.  I often 
leave blocks on for a long time and this seems to help or at least not
hurt.

Thanks,
Anna
-- 
Anna K. Schultz
SDRC Core A Research Technician
Department of Dermatology
College of Physicians and Surgeons
Columbia University
Vanderbilt Clinic 15-211
630 West 168^th Street
New York, NY 10032
Phone: (212) 305-4954

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