I have just spent the last few hours doing frozen sections on a number
of clinical skin specimens using the procedure that Stephen is talking
about. I have been using this approach for well over a year and I love
it!! It solves most of the problems routinely associated with doing
frozens on skin.The quality of the sections is great, the orientation is
quick and easy, and the overall time required is less.
Stephen has obviously put a lot of thought and experience into the
development of this system ... and it shows. When I first saw the video
clip on his web site, I was was skeptical and thought it all looked too
easy. I was wrong; it really is that easy!
Sure, there is some expense. You need to buy the freezing blocks, heat
extractor blocks, and chucks (in a pinch, you can actually use the
Microm chucks too). But, if quality of section, ease of use, and time
saving are important ... you owe it to yourself to give it a try.
I am very surprised to hear that the cryostat manufacturers are not
promoting this system. What is there to lose? If a vendor has
complained about this system being promoted on the Histonet, too bad!
The prime purpose of the Histonet is to provide a forum for
histotechnologists, pathologists, researchers, etc. to share
information, seek advise, or share experiences.
The Histonet is not a forum for blatant commercial advertising, but I am
very impressed with this system and would recommend it to anyone who
does frozen sections. The website is well put together and well worth
Stephen Peters M.D. wrote:
> For the last 5 years I have been promoting a system of face down embedding tissue
> for frozen section which I developed over my 25 year surgical pathology practice.
> The system is rapid, very precise and wastes very little tissue. I am all but exhausted devoting most of my vacation and free time to bring this sytem to my colleagues at very limited profit.My chucks are made y.to be used cold with deep wide
> waffle pattern grooves. They are made of stainless so they function as a heat extractor.
> With the exception of Leica, I am quite surprised and saddened by the lack of interest in
> our cryostat manufacturers to explore a new technology which could benefit their customers. I believe they are hoping I fade away. If you want to improve your frozen section practice, I suggest you seek out this system. I would be happy to direct you to it, but the last time I offered help, one of the vendors complained I was breaking a vendor rule.
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